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ELK Biotechnology
SKU:ES6833
MEK-7 (phospho Ser271) Rabbit Polyclonal Antibody
MEK-7 (phospho Ser271) Rabbit Polyclonal Antibody
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MEK-7 (phospho Ser271) Rabbit Polyclonal Antibody
Overview
| Product name: | MEK-7 (phospho Ser271) rabbit pAb |
| Alternative Names: | MAP2K7; JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; S |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Ser271. AA range:236-285 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 47kD |
| GeneID(Human): | 5609 |
| Human Swiss-Prot No: | O14733 |
| Cellular localization: | Nucleus. Cytoplasm . |
| Background: | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Ser271) Antibody -
Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K7 (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from NIH/3T3 cells treated with insulin 0.01U/ml 15' and NIH/3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is blocked with the phospho peptide.
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