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ELK Biotechnology
SKU:ES6817
ERK 3 Rabbit Polyclonal Antibody
ERK 3 Rabbit Polyclonal Antibody
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$248.00 USD
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ERK 3 Rabbit Polyclonal Antibody
Overview
| Product name: | ERK 3 rabbit pAb |
| Alternative Names: | MAPK6; ERK3; PRKM6; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p97 MAPK. AA range:461-510 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 85kD |
| GeneID(Human): | 5597 |
| Human Swiss-Prot No: | Q16659 |
| Cellular localization: | Cytoplasm . Nucleus . Translocates to the cytoplasm following interaction with MAPKAPK5. . |
| Background: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western blot analysis of Hela Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Immunofluorescence analysis of MCF7 cells, using p97 MAPK Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from K562 cells, using p97 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.
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