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ELK Biotechnology
SKU:ES6791
PBK (phospho Thr9) Rabbit Polyclonal Antibody
PBK (phospho Thr9) Rabbit Polyclonal Antibody
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PBK (phospho Thr9) Rabbit Polyclonal Antibody
Overview
| Product name: | PBK (phospho Thr9) rabbit pAb |
| Alternative Names: | PBK; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinas |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PBK/TOPK around the phosphorylation site of Thr9. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 36kD |
| GeneID(Human): | 55872 |
| Human Swiss-Prot No: | Q96KB5 |
| Cellular localization: | nucleus, |
| Background: | PDZ binding kinase(PBK) Homo sapiens This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of 293T cells using Phospho-PBK (T9) Polyclonal Antibody diluted at 1:500 -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PBK/TOPK (Phospho-Thr9) Antibody -
Western blot analysis of lysates from K562 cells treated with UV 30', using PBK/TOPK (Phospho-Thr9) Antibody. The lane on the right is blocked with the phospho peptide. -
Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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