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ELK Biotechnology
SKU:ES6789
PKD1/2/3 (phospho Ser738/S742) Rabbit Polyclonal Antibody
PKD1/2/3 (phospho Ser738/S742) Rabbit Polyclonal Antibody
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PKD1/2/3 (phospho Ser738/S742) Rabbit Polyclonal Antibody
Overview
| Product name: | PKD1/2/3 (phospho Ser738/S742) rabbit pAb |
| Alternative Names: | PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu; PRKD2; PKD2; HSPC187; Serine/threonine-protein kinase D2; nPKC-D2; PRKD3; EPK2; PRKCN; Serine/threonine-protein kinas |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKD1/2/3/PKC mu around the phosphorylation site of Ser738 and Ser742. AA range:706-755 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 101kD |
| GeneID(Human): | 5587/25865/23683 |
| Human Swiss-Prot No: | Q15139/Q9BZL6/O94806 |
| Cellular localization: | Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. |
| Background: | PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of A549 cells using Phospho-PKD1/2/3 (S738/S742) Polyclonal Antibody -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody -
Immunohistochemistry analysis of paraffin-embedded human heart, using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30' and HT29 cells treated with serum 20% 15', using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The lane on the right is blocked with the phospho peptide.
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