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ELK Biotechnology
SKU:ES6787
PKD1 (phospho Tyr463) Rabbit Polyclonal Antibody
PKD1 (phospho Tyr463) Rabbit Polyclonal Antibody
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PKD1 (phospho Tyr463) Rabbit Polyclonal Antibody
Overview
| Product name: | PKD1 (phospho Tyr463) rabbit pAb |
| Alternative Names: | PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu around the phosphorylation site of Tyr463. AA range:429-478 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 130kD |
| GeneID(Human): | 5587 |
| Human Swiss-Prot No: | Q15139 |
| Cellular localization: | Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. |
| Background: | PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKD1/PKC mu (Phospho-Tyr463) Antibody -
Immunohistochemistry analysis of paraffin-embedded human brain, using PKD1/PKC mu (Phospho-Tyr463) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from HepG2 cells, using PKD1/PKC mu (Phospho-Tyr463) Antibody. The lane on the right is blocked with the phospho peptide.
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