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ELK Biotechnology
SKU:ES6685
TRAC-1 Rabbit Polyclonal Antibody
TRAC-1 Rabbit Polyclonal Antibody
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TRAC-1 Rabbit Polyclonal Antibody
Overview
| Product name: | TRAC-1 rabbit pAb |
| Alternative Names: | RNF125; E3 ubiquitin-protein ligase RNF125; RING finger protein 125; T-cell RING activation protein 1; TRAC-1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RNF125. AA range:131-180 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 26kD |
| GeneID(Human): | 54941 |
| Human Swiss-Prot No: | Q96EQ8 |
| Cellular localization: | Golgi apparatus membrane ; Lipid-anchor . Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane. . |
| Background: | ring finger protein 125(RNF125) Homo sapiens This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012], |
| Species Reactivity: | Human;Mouse;Rat |
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Western blot analysis of lysates from K562 and HT-29 cells, using RNF125 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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