MRPL39; C21orf92; MRPL5; RPML5; MSTP003; PRED22; 39S ribosomal protein L39; mitochondrial; L39mt; MRP-L39; 39S ribosomal protein L5, mitochondrial; L5mt; MRP-L5
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human MRPL39. AA range:289-338
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
39kD
GeneID（Human）:
54148
Human Swiss-Prot No:
Q9NYK5
Cellular localization:
Mitochondrion .
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Two transcript variants encoding distinct isoforms have been described. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Jul 2008],
Western Blot analysis of 3T3 cells using MRP-L39 Polyclonal Antibody diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using MRPL39 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat and MCF-7 cells, using MRPL39 Antibody. The lane on the right is blocked with the synthesized peptide.