PEG3; KIAA0287; ZSCAN24; Paternally-expressed gene 3 protein; Zinc finger and SCAN domain-containing protein 24
Applications:
WB;IHC
Recommended Dilutions:
WB 1:500-2000;IHC-p 1:50-300
Immunogen:
The antiserum was produced against synthesized peptide derived from human PEG3. AA range:1031-1080
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
181kD
GeneID（Human）:
5178
Human Swiss-Prot No:
Q9GZU2
Cellular localization:
Nucleus . Cytoplasm .
Background:
In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009],
Western Blot analysis of A549 cells using PEG3 Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).