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ELK Biotechnology
SKU:ES6565
PEDF Rabbit Polyclonal Antibody
PEDF Rabbit Polyclonal Antibody
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$248.00 USD
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PEDF Rabbit Polyclonal Antibody
Overview
| Product name: | PEDF rabbit pAb |
| Alternative Names: | SERPINF1; PEDF; PIG35; Pigment epithelium-derived factor; PEDF; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1 |
| Applications: | IHC;IF;WB;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PEDF. AA range:258-307 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 46kD |
| GeneID(Human): | 5176 |
| Human Swiss-Prot No: | P36955 |
| Cellular localization: | Secreted . Melanosome . Enriched in stage I melanosomes. |
| Background: | This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. -
Immunohistochemistry analysis of PEDF antibody in paraffin-embedded human prostate carcinoma tissue. -
Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night
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