NOTCH4; INT3; Neurogenic locus notch homolog protein 4; Notch 4; hNotch4
Applications:
WB;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human NOTCH4. AA range:1401-1450
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
59kD
GeneID（Human）:
4855
Human Swiss-Prot No:
Q99466
Cellular localization:
Cell membrane; Single-pass type I membrane protein.; [Notch 4 intracellular domain]: Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
Background:
notch 4(NOTCH4) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which
Western Blot analysis of A549 NIH-3T3 SH-SY5Y K562 HELA cells using Cleaved-Notch 4 (V1432) Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from 293 and COS7 cells, treated with etoposide 25uM 1h, using NOTCH4 (Cleaved-Val1432) Antibody. The lane on the right is blocked with the synthesized peptide.