1
/
of
1
ELK Biotechnology
SKU:ES6400
NOS3 (phospho Ser615) Rabbit Polyclonal Antibody
NOS3 (phospho Ser615) Rabbit Polyclonal Antibody
Regular price
$248.00 USD
Regular price
Sale price
$248.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
NOS3 (phospho Ser615) Rabbit Polyclonal Antibody
Overview
| Product name: | eNOS (phospho Ser615) rabbit pAb |
| Alternative Names: | NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII |
| Applications: | WB;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser615. AA range:581-630 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 130-140kD |
| GeneID(Human): | 4846 |
| Human Swiss-Prot No: | P29474 |
| Cellular localization: | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| Background: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009], |
| Species Reactivity: | Human;Mouse;Rat |
-
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eNOS (Phospho-Ser615) Antibody -
Western blot analysis of lysates from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody. The lane on the right is blocked with the phospho peptide.
Share
