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ELK Biotechnology
SKU:ES6387
IκB-ε Rabbit Polyclonal Antibody
IκB-ε Rabbit Polyclonal Antibody
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IκB-ε Rabbit Polyclonal Antibody
Overview
| Product name: | IκB-ε rabbit pAb |
| Alternative Names: | NFKBIE; IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon. AA range:131-180 |
| Storage: | -20°C/1 year |
| Storage: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 45kD |
| GeneID(Human): | 4794 |
| Human Swiss-Prot No: | O00221 |
| Cellular localization: | Cytoplasm . |
| Background: | The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon Antibody. The picture on the right is blocked with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IkappaB-epsilon Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 5', using IkappaB-epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
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