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ELK Biotechnology
SKU:ES6384
IκB-β (phospho Thr19) Rabbit Polyclonal Antibody
IκB-β (phospho Thr19) Rabbit Polyclonal Antibody
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IκB-β (phospho Thr19) Rabbit Polyclonal Antibody
Overview
| Product name: | IκB-β (phospho Thr19) rabbit pAb |
| Alternative Names: | NFKBIB; IKBB; TRIP9; NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Thr19. AA range:4-53 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 37kD |
| GeneID(Human): | 4793 |
| Human Swiss-Prot No: | Q15653 |
| Cellular localization: | Cytoplasm . Nucleus . |
| Background: | The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of 293 cells using Phospho-IκB-β (T19) Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 30', using IkappaB-beta (Phospho-Thr19) Antibody. The lane on the right is blocked with the phospho peptide.
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