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ELK Biotechnology
SKU:ES6329
ATF-3 Rabbit Polyclonal Antibody
ATF-3 Rabbit Polyclonal Antibody
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ATF-3 Rabbit Polyclonal Antibody
Overview
| Product name: | ATF-3 rabbit pAb |
| Alternative Names: | ATF3; Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ATF3. AA range:131-180 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 21kD |
| GeneID(Human): | 467 |
| Human Swiss-Prot No: | P18847 |
| Cellular localization: | Nucleus . |
| Background: | activating transcription factor 3(ATF3) Homo sapiens This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of 293T-UV cells using ATF-3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from RAW264.7 cells, using ATF3 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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