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ELK Biotechnology
SKU:ES6302
B-Myb (phospho Ser577) Rabbit Polyclonal Antibody
B-Myb (phospho Ser577) Rabbit Polyclonal Antibody
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B-Myb (phospho Ser577) Rabbit Polyclonal Antibody
Overview
| Product name: | B-Myb (phospho Ser577) rabbit pAb |
| Alternative Names: | MYBL2; BMYB; Myb-related protein B; B-Myb; Myb-like protein 2 |
| Applications: | WB;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human B-Myb around the phosphorylation site of Ser577/581. AA range:551-600 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 80kD |
| GeneID(Human): | 4605 |
| Human Swiss-Prot No: | P10244 |
| Cellular localization: | Nucleus. |
| Background: | The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013], |
| Species Reactivity: | Human;Mouse;Rat |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-Myb (Phospho-Ser577/581) Antibody -
Immunofluorescence analysis of HeLa cells, using B-Myb (Phospho-Ser577/581) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from K562 cells, using B-Myb (Phospho-Ser577/581) Antibody. The lane on the right is blocked with the phospho peptide.
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