MAP3K9; MLK1; PRKE1; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1; MAP3K10; MLK2; MST; Mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; Protein kinase MST
Applications:
IHC;IF;ELISA
Recommended Dilutions:
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266. AA range:281-330
Storage:
-20°C/1 year
Storage:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
GeneID（Human）:
4293/4294
Human Swiss-Prot No:
P80192/Q02779
Cellular localization:
intracellular,integral component of membrane,
Background:
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Homodimer.,tissue specificity:Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.,
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MLK1/2 (Phospho-Thr312/266) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using MLK1/2 (Phospho-Thr312/266) Antibody. The picture on the right is blocked with the phospho peptide.