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ELK Biotechnology
SKU:ES6223
MEF-2C Rabbit Polyclonal Antibody
MEF-2C Rabbit Polyclonal Antibody
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MEF-2C Rabbit Polyclonal Antibody
Overview
| Product name: | MEF-2C rabbit pAb |
| Alternative Names: | MEF2C; Myocyte-specific enhancer factor 2C |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MEF2C. AA range:362-411 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 51kD |
| GeneID(Human): | 4208 |
| Human Swiss-Prot No: | Q06413 |
| Cellular localization: | Nucleus . Cytoplasm, sarcoplasm . |
| Background: | This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of 293T cells using MEF-2C Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from NIH/3T3 cells, treated with starved 24h, using MEF2C Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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