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ELK Biotechnology
SKU:ES6205
Mcl-1 (phospho Ser159) Rabbit Polyclonal Antibody
Mcl-1 (phospho Ser159) Rabbit Polyclonal Antibody
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Mcl-1 (phospho Ser159) Rabbit Polyclonal Antibody
Overview
| Product name: | Mcl-1 (phospho Ser159) rabbit pAb |
| Alternative Names: | MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MCL1 around the phosphorylation site of Ser159. AA range:125-174 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | About 40kd in human,39kd in mouse and rat |
| GeneID(Human): | 4170 |
| Human Swiss-Prot No: | Q07820 |
| Cellular localization: | Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria. |
| Background: | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010], |
| Species Reactivity: | Human;Mouse |
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Western blot analysis of K562 using p-Mcl-1 (S159) antibody. -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MCL1 (Phospho-Ser159) Antibody -
Immunofluorescence analysis of A549 cells, using MCL1 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide. -
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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