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ELK Biotechnology
SKU:ES6121
HSL (phospho Ser552) Rabbit Polyclonal Antibody
HSL (phospho Ser552) Rabbit Polyclonal Antibody
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HSL (phospho Ser552) Rabbit Polyclonal Antibody
Overview
| Product name: | HSL (phospho Ser552) rabbit pAb |
| Alternative Names: | LIPE; Hormone-sensitive lipase; HSL |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser552. AA range:518-567 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 117kD |
| GeneID(Human): | 3991 |
| Human Swiss-Prot No: | Q05469 |
| Cellular localization: | Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity). . |
| Background: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of 293 cells using Phospho-HSL (S552) Polyclonal Antibody diluted at 1:500 -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSL (Phospho-Ser552) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from K562 cells, using HSL (Phospho-Ser552) Antibody. The lane on the right is blocked with the phospho peptide.
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