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ELK Biotechnology
SKU:ES6120
HSL (phospho Ser855) Rabbit Polyclonal Antibody
HSL (phospho Ser855) Rabbit Polyclonal Antibody
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HSL (phospho Ser855) Rabbit Polyclonal Antibody
Overview
| Product name: | HSL (phospho Ser855) rabbit pAb |
| Alternative Names: | LIPE; Hormone-sensitive lipase; HSL |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554. AA range:520-569 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 81kD |
| GeneID(Human): | 3991 |
| Human Swiss-Prot No: | Q05469 |
| Cellular localization: | Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity). . |
| Background: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western blot analysis of L929 using p-HSL (S855) antibody. Antibody was diluted at 1:1000 -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSL (Phospho-Ser855/554) Antibody -
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ng/ml 24h, using HSL (Phospho-Ser855/554) Antibody. The lane on the right is blocked with the phospho peptide.
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