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ELK Biotechnology
SKU:ES6106
Rho GDIα (phospho Ser174) Rabbit Polyclonal Antibody
Rho GDIα (phospho Ser174) Rabbit Polyclonal Antibody
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Rho GDIα (phospho Ser174) Rabbit Polyclonal Antibody
Overview
| Product name: | Rho GDIα (phospho Ser174) rabbit pAb |
| Alternative Names: | ARHGDIA; GDIA1; Rho GDP-dissociation inhibitor 1; Rho GDI 1; Rho-GDI alpha |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ARHGDIA around the phosphorylation site of Ser174. AA range:140-189 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 23kD |
| GeneID(Human): | 396 |
| Human Swiss-Prot No: | P52565 |
| Cellular localization: | Cytoplasm . |
| Background: | This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Species Reactivity: | Human;Mouse;Rat |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ARHGDIA (Phospho-Ser174) Antibody -
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma, using ARHGDIA (Phospho-Ser174) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using ARHGDIA (Phospho-Ser174) Antibody. The lane on the right is blocked with the phospho peptide.
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