1
/
of
1
ELK Biotechnology
SKU:ES6077
Laminin α-5 Rabbit Polyclonal Antibody
Laminin α-5 Rabbit Polyclonal Antibody
Regular price
$248.00 USD
Regular price
Sale price
$248.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Laminin α-5 Rabbit Polyclonal Antibody
Overview
| Product name: | Laminin α-5 rabbit pAb |
| Alternative Names: | LAMA5; KIAA0533; KIAA1907; Laminin subunit alpha-5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human LAMA5. AA range:2381-2430 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 400kD |
| GeneID(Human): | 3911 |
| Human Swiss-Prot No: | O15230 |
| Cellular localization: | Secreted, extracellular space, extracellular matrix, basement membrane. Major component. |
| Background: | This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013], |
| Species Reactivity: | Human;Mouse |
-
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. -
Western Blot analysis of RAW HepG2 A549 cells using Laminin α-5 Polyclonal Antibody -
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LAMA5 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night
Share
