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ELK Biotechnology
SKU:ES5976
JAK3 Rabbit Polyclonal Antibody
JAK3 Rabbit Polyclonal Antibody
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$248.00 USD
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JAK3 Rabbit Polyclonal Antibody
Overview
| Product name: | JAK3 rabbit pAb |
| Alternative Names: | JAK3; Tyrosine-protein kinase JAK3; Janus kinase 3; JAK-3; Leukocyte janus kinase; L-JAK |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human JAK3. AA range:751-800 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 125kD |
| GeneID(Human): | 3718 |
| Human Swiss-Prot No: | P52333 |
| Cellular localization: | Endomembrane system ; Peripheral membrane protein . Cytoplasm . |
| Background: | The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,JAK3 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. -
Immunofluorescence analysis of human-uterus tissue. 1,JAK3 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Pictur -
Immunofluorescence analysis of rat-kidney tissue. 1,JAK3 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture -
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,JAK3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tem
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