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ELK Biotechnology
SKU:ES5960
Emt (phospho Tyr512) Rabbit Polyclonal Antibody
Emt (phospho Tyr512) Rabbit Polyclonal Antibody
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Emt (phospho Tyr512) Rabbit Polyclonal Antibody
Overview
| Product name: | Emt (phospho Tyr512) rabbit pAb |
| Alternative Names: | ITK; EMT; LYK; Tyrosine-protein kinase ITK/TSK; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. AA range:478-527 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 72kD |
| GeneID(Human): | 3702 |
| Human Swiss-Prot No: | Q08881 |
| Cellular localization: | Cytoplasm . Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. |
| Background: | This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western blot analysis of 293 using p-Emt (Y512) antibody. -
Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by
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