A-Raf (phospho Tyr302) Rabbit Polyclonal Antibody

Name: A-Raf (phospho Tyr302) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES5953
Price: 248.0 USD
Availability: InStock

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A-Raf (phospho Tyr302) Rabbit Polyclonal Antibody

Overview

Product name:	A-Raf (phospho Tyr302) rabbit pAb
Alternative Names:	ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302. AA range:276-325
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	68kD
GeneID（Human）:	369
Human Swiss-Prot No:	P10398
Cellular localization:	intracellular,mitochondrion,cytosol,
Background:	This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],
Species Reactivity:	Human;Mouse;Rat

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using A-RAF (Phospho-Tyr302) Antibody
Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right is blocked with the phospho peptide.

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A-Raf (phospho Tyr302) Rabbit Polyclonal Antibody

A-Raf (phospho Tyr302) Rabbit Polyclonal Antibody

Overview