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ELK Biotechnology
SKU:ES5928
IRS-1 (phospho Ser323) Rabbit Polyclonal Antibody
IRS-1 (phospho Ser323) Rabbit Polyclonal Antibody
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IRS-1 (phospho Ser323) Rabbit Polyclonal Antibody
Overview
| Product name: | IRS-1 (phospho Ser323) rabbit pAb |
| Alternative Names: | IRS1; Insulin receptor substrate 1; IRS-1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser323. AA range:289-338 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 170kD |
| GeneID(Human): | 3667 |
| Human Swiss-Prot No: | P35568 |
| Cellular localization: | nucleus,cytoplasm,cytosol,plasma membrane,insulin receptor complex,caveola,intracellular membrane-bounded organelle, |
| Background: | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009], |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Western blot analysis of 293T using p-IRS-1 (S323) antibody. Antibody was diluted at 1:1000 -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser323) Antibody -
Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide.
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