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ELK Biotechnology
SKU:ES5865
IKKβ (phospho Tyr188) Rabbit Polyclonal Antibody
IKKβ (phospho Tyr188) Rabbit Polyclonal Antibody
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IKKβ (phospho Tyr188) Rabbit Polyclonal Antibody
Overview
| Product name: | IKKβ (phospho Tyr188) rabbit pAb |
| Alternative Names: | IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr188. AA range:161-210 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 86kD |
| GeneID(Human): | 3551 |
| Human Swiss-Prot No: | O14920 |
| Cellular localization: | Cytoplasm . Nucleus . Membrane raft . Colocalized with DPP4 in membrane rafts. . |
| Background: | The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011], |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-beta (Phospho-Tyr188) Antibody -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-beta (Phospho-Tyr188) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from COS7 cells, using IKK-beta (Phospho-Tyr188) Antibody. The lane on the right is blocked with the phospho peptide.
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