Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human IGF2R around the phosphorylation site of Ser2409. AA range:2381-2430
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
300kD
GeneID（Human）:
3482
Human Swiss-Prot No:
P11717
Cellular localization:
Golgi apparatus membrane ; Single-pass type I membrane protein . Endosome membrane ; Single-pass type I membrane protein . Mainly localized in the Golgi at steady state and not detectable in lysosome (PubMed:18817523). Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface (PubMed:10900005). .
Background:
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611). [provided by RefSeq, Nov 2015],
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IGF2R (Phospho-Ser2409) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using IGF2R (Phospho-Ser2409) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COS7 cells treated with UV 15', using IGF2R (Phospho-Ser2409) Antibody. The lane on the right is blocked with the phospho peptide.