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ELK Biotechnology
SKU:ES5740
HXK III Rabbit Polyclonal Antibody
HXK III Rabbit Polyclonal Antibody
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$248.00 USD
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HXK III Rabbit Polyclonal Antibody
Overview
| Product name: | HXK III rabbit pAb |
| Alternative Names: | HK3; Hexokinase-3; Hexokinase type III; HK III |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Hexokinase-3. AA range:811-860 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 99kD |
| GeneID(Human): | 3101 |
| Human Swiss-Prot No: | P52790 |
| Cellular localization: | cell,mitochondrion,cytosol, |
| Background: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western blot analysis of lysates from Jurkat cells, treated with insulin 0.01U/ml 15', using Hexokinase-3 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from COLO205 cells using Hexokinase-3 antibody. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200
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