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ELK Biotechnology
SKU:ES5737
Hint1 Rabbit Polyclonal Antibody
Hint1 Rabbit Polyclonal Antibody
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$248.00 USD
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Hint1 Rabbit Polyclonal Antibody
Overview
| Product name: | Hint1 rabbit pAb |
| Alternative Names: | HINT1; HINT; PKCI1; PRKCNH1; Histidine triad nucleotide-binding protein 1; Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HINT1. AA range:71-120 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 28kD |
| GeneID(Human): | 3094 |
| Human Swiss-Prot No: | P49773 |
| Cellular localization: | Cytoplasm . Nucleus . Interaction with CDK7 leads to a more nuclear localization. . |
| Background: | This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-abs -
Immunofluorescence analysis of HeLa cells, using HINT1 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from Jurkat cells, using HINT1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HepG2 cells using HINT1 antibody.
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