HDAC1 (phospho Ser421) Rabbit Polyclonal Antibody

Name: HDAC1 (phospho Ser421) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES5729
Price: 248.0 USD
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HDAC1 (phospho Ser421) Rabbit Polyclonal Antibody

Overview

Product name:	HDAC1 (phospho Ser421) rabbit pAb
Alternative Names:	HDAC1; RPD3L1; Histone deacetylase 1; HD1
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human HDAC1 around the phosphorylation site of Ser421. AA range:387-436
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	62kD
GeneID（Human）:	3065
Human Swiss-Prot No:	Q13547
Cellular localization:	Nucleus .
Background:	Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008],
Species Reactivity:	Human;Mouse;Rat

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HDAC1 (Phospho-Ser421) Antibody
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using HDAC1 (Phospho-Ser421) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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HDAC1 (phospho Ser421) Rabbit Polyclonal Antibody

HDAC1 (phospho Ser421) Rabbit Polyclonal Antibody

Overview