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ELK Biotechnology
SKU:ES5688
TFIIH p44 Rabbit Polyclonal Antibody
TFIIH p44 Rabbit Polyclonal Antibody
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$248.00 USD
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TFIIH p44 Rabbit Polyclonal Antibody
Overview
| Product name: | TFIIH p44 rabbit pAb |
| Alternative Names: | GTF2H2; BTF2P44; General transcription factor IIH subunit 2; Basic transcription factor 2 44 kDa subunit; BTF2 p44; General transcription factor IIH polypeptide 2; TFIIH basal transcription factor complex p44 subunit |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TF2H2. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 62kD |
| GeneID(Human): | 2966 |
| Human Swiss-Prot No: | Q13888 |
| Cellular localization: | Nucleus . |
| Background: | This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of t |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of COLO205 cells using TFIIH p44 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from COLO205 cells, using TF2H2 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from COLO205 cells using TF2H2 antibody. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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