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ELK Biotechnology
SKU:ES5671
MRP-L22 Rabbit Polyclonal Antibody
MRP-L22 Rabbit Polyclonal Antibody
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MRP-L22 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-L22 rabbit pAb |
| Alternative Names: | MRPL22; MRPL25; RPML25; HSPC158; 39S ribosomal protein L22; mitochondrial; L22mt; MRP-L22; 39S ribosomal protein L25, mitochondrial; L25mt; MRP-L25 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPL22. AA range:91-140 |
| Storage: | -20°C/1 year |
| Storage: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 24kD |
| GeneID(Human): | 29093 |
| Human Swiss-Prot No: | Q9NWU5 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provide |
| Species Reactivity: | Human;Mouse;Rat |
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Western blot analysis of lysates from HepG2 cells, using MRPL22 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from Jurkat cells using MRPL22 antibody. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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