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ELK Biotechnology
SKU:ES5546
Arfaptin 1 Rabbit Polyclonal Antibody
Arfaptin 1 Rabbit Polyclonal Antibody
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$248.00 USD
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Arfaptin 1 Rabbit Polyclonal Antibody
Overview
| Product name: | Arfaptin 1 rabbit pAb |
| Alternative Names: | ARFIP1; Arfaptin-1; ADP-ribosylation factor-interacting protein 1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ARFIP1. AA range:271-320 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 40kD |
| GeneID(Human): | 27236 |
| Human Swiss-Prot No: | P53367 |
| Cellular localization: | Golgi apparatus . Golgi apparatus, trans-Golgi network membrane . |
| Background: | function:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart., |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorb -
Immunofluorescence analysis of A549 cells, using ARFIP1 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from Jurkat cells, using ARFIP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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