HBP1 (phospho Ser402) Rabbit Polyclonal Antibody

Name: HBP1 (phospho Ser402) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES5519
Price: 248.0 USD
Availability: InStock

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HBP1 (phospho Ser402) Rabbit Polyclonal Antibody

Overview

Product name:	HBP1 (phospho Ser402) rabbit pAb
Alternative Names:	HBP1; HMG box-containing protein 1; HMG box transcription factor 1; High mobility group box transcription factor 1
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human HBP1 around the phosphorylation site of Ser402. AA range:371-420
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	58kD
GeneID（Human）:	26959
Human Swiss-Prot No:	O60381
Cellular localization:	Nucleus .
Background:	function:Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.,similarity:Contains 1 AXH domain.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the second PAH repeat of SIN3A (By similarity). Binds TCF4 and RB1.,
Species Reactivity:	Human;Rat;Mouse;

Western Blot analysis of A549 cells using Phospho-HBP1 (S402) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor
Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30', using HBP1 (Phospho-Ser402) Antibody. The lane on the right is blocked with the phospho peptide.

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HBP1 (phospho Ser402) Rabbit Polyclonal Antibody

HBP1 (phospho Ser402) Rabbit Polyclonal Antibody

Overview