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ELK Biotechnology
SKU:ES5280
TPX2 Rabbit Polyclonal Antibody
TPX2 Rabbit Polyclonal Antibody
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$248.00 USD
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TPX2 Rabbit Polyclonal Antibody
Overview
| Product name: | TPX2 rabbit pAb |
| Alternative Names: | TPX2; C20orf1; C20orf2; DIL2; HCA519; Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Protein fls353; Restricted expression prolifera |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DIL-2. AA range:301-350 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 86kD |
| GeneID(Human): | 22974 |
| Human Swiss-Prot No: | Q9ULW0 |
| Cellular localization: | Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. . |
| Background: | developmental stage:Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,subcellular location:During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus.,tissue specificity:Expressed in lung carcinoma cell lines but not in normal lung tissues., |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of Jurkat cells using TPX2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DIL-2 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from Jurkat cells, using DIL-2 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HUVECcells using DIL-2 antibody.
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