Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human HER3 around the phosphorylation site of Tyr1197. AA range:1163-1212
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
160-170kD
GeneID（Human）:
2065
Human Swiss-Prot No:
P21860
Cellular localization:
[Isoform 1]: Cell membrane ; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
Background:
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the m
Western Blot analysis of 293T cells using Phospho-ErbB-3 (Y1197) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from HepG2 cells , using HER3 (Phospho-Tyr1197) Antibody. The lane on the right is blocked with the phospho peptide.