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ELK Biotechnology
SKU:ES5088
Elf-1 Rabbit Polyclonal Antibody
Elf-1 Rabbit Polyclonal Antibody
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Elf-1 Rabbit Polyclonal Antibody
Overview
| Product name: | Elf-1 rabbit pAb |
| Alternative Names: | ELF1; ETS-related transcription factor Elf-1; E74-like factor 1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ELF1. AA range:537-586 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 67kD |
| GeneID(Human): | 1997 |
| Human Swiss-Prot No: | P32519 |
| Cellular localization: | Nucleus. |
| Background: | This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009], |
| Species Reactivity: | Human |
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Western Blot analysis of 293 cells using Elf-1 Polyclonal Antibody diluted at 1:1000 -
Immunofluorescence analysis of A549 cells, using ELF1 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from 293 and HUVEC cells, using ELF1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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