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ELK Biotechnology
SKU:ES4970
Elongin A3 Rabbit Polyclonal Antibody
Elongin A3 Rabbit Polyclonal Antibody
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$248.00 USD
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Elongin A3 Rabbit Polyclonal Antibody
Overview
| Product name: | Elongin A3 rabbit pAb |
| Alternative Names: | TCEB3C; TCEB3L2; RNA polymerase II transcription factor SIII subunit A3; Elongin-A3; EloA3; Transcription elongation factor B polypeptide 3C |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ELOA3. AA range:381-430 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 60kD |
| GeneID(Human): | 162699 |
| Human Swiss-Prot No: | Q8NG57 |
| Cellular localization: | integral component of membrane,elongin complex, |
| Background: | The SIII (or elongin) transcription elongation factor complex stimulates the rate of transcription elongation by RNA polymerase II by suppressing the transient pausing of the polymerase at many sites along the DNA template. This complex is a heterotrimer, composed of the transcriptionally active subunit A, A2 or A3 (or elongin A, A2 or A3) and two regulatory subunits, B and C (or elongin B and C). This gene encodes subunit A3. A3 and A are ubiquitously expressed, whereas A2 is specifically expressed in the testis. [provided by RefSeq, Mar 2010], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of COLO205 cells using Elongin A3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from COLO205 cells, using ELOA3 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from COLO205 cells using CIDEB antibody. -
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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