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ELK Biotechnology
SKU:ES4967
DAPK3 (phospho Thr265) Rabbit Polyclonal Antibody
DAPK3 (phospho Thr265) Rabbit Polyclonal Antibody
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DAPK3 (phospho Thr265) Rabbit Polyclonal Antibody
Overview
| Product name: | DAPK3 (phospho Thr265) rabbit pAb |
| Alternative Names: | DAPK3; ZIPK; Death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 52kD |
| GeneID(Human): | 1613 |
| Human Swiss-Prot No: | O43293 |
| Cellular localization: | Nucleus . Cytoplasm . Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. .; [Isoform 1]: Nucleus . Cytoplasm .; [Isoform 2]: Nucleus . Cytoplasm . |
| Background: | Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,DAPK3 (phospho Thr265) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-tubulin Monoclonal Antibody(8F11)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS36 -
Western Blot analysis of HuvEc cells using Phospho-DAPK3 (T265) Polyclonal Antibody -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK3 (Phospho-Thr265) Antibody -
Immunofluorescence analysis of A549 cells, using DAPK3 (Phospho-Thr265) Antibody. The picture on the right is blocked with the phospho peptide.
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