Casein Kinase IIα (phospho Tyr255) Rabbit Polyclonal Antibody

Name: Casein Kinase IIα (phospho Tyr255) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES4863
Price: 248.0 USD
Availability: InStock

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Casein Kinase IIα (phospho Tyr255) Rabbit Polyclonal Antibody

Overview

Product name:	Casein Kinase IIα (phospho Tyr255) rabbit pAb
Alternative Names:	CSNK2A1; CK2A1; Casein kinase II subunit alpha; CK II alpha
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Casein Kinase II alpha around the phosphorylation site of Tyr255. AA range:221-270
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	45kD
GeneID（Human）:	1457
Human Swiss-Prot No:	P68400
Cellular localization:	Nucleus .
Background:	Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq, Jul 2014],
Species Reactivity:	Human;Mouse;Rat

Western Blot analysis of HeLa cells using Phospho-Casein Kinase IIα (Y255) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Casein Kinase II alpha (Phospho-Tyr255) Antibody
Western blot analysis of lysates from HeLa cells and Jurkat cells, using Casein Kinase II alpha (Phospho-Tyr255) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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Casein Kinase IIα (phospho Tyr255) Rabbit Polyclonal Antibody

Casein Kinase IIα (phospho Tyr255) Rabbit Polyclonal Antibody

Overview