Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Claudin 7. AA range:162-211
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
32kD
GeneID（Human）:
1366
Human Swiss-Prot No:
O95471
Cellular localization:
Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane . Cell junction, tight junction . Co-localizes with EPCAM at the basolateral cell membrane and tight junction. .
Background:
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010],
Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using Claudin 7 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from rat liver cells, using Claudin 7 Antibody. The lane on the right is blocked with the synthesized peptide.