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ELK Biotechnology
SKU:ES4615
Frizzled-10 Rabbit Polyclonal Antibody
Frizzled-10 Rabbit Polyclonal Antibody
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Frizzled-10 Rabbit Polyclonal Antibody
Overview
| Product name: | Frizzled-10 rabbit pAb |
| Alternative Names: | FZD10; Frizzled-10; Fz-10; hFz10; FzE7; CD antigen CD350 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human FZD10. AA range:135-184 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 60kD |
| GeneID(Human): | 11211 |
| Human Swiss-Prot No: | Q9ULW2 |
| Cellular localization: | Cell membrane ; Multi-pass membrane protein . |
| Background: | This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Monkey |
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Western Blot analysis of COS-7 cells using Frizzled-10 Polyclonal Antibody -
Western blot analysis of lysates from COS7 cells, using FZD10 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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