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ELK Biotechnology
SKU:ES4585
Stonin-1 Rabbit Polyclonal Antibody
Stonin-1 Rabbit Polyclonal Antibody
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Stonin-1 Rabbit Polyclonal Antibody
Overview
| Product name: | Stonin-1 rabbit pAb |
| Alternative Names: | STON1; SALF; SBLF; STN1; Stonin-1; Stoned B-like factor |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human STON1. AA range:686-735 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 83kD |
| GeneID(Human): | 11037 |
| Human Swiss-Prot No: | Q9Y6Q2 |
| Cellular localization: | Cytoplasm . Membrane . Some fraction is membrane-associated. |
| Background: | stonin 1(STON1) Homo sapiens Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of K562 cells using Stonin-1 Polyclonal Antibody -
Western blot analysis of lysates from K562 cells, using STON1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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