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ELK Biotechnology
SKU:ES4579
KIF2C (phospho Ser95) Rabbit Polyclonal Antibody
KIF2C (phospho Ser95) Rabbit Polyclonal Antibody
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KIF2C (phospho Ser95) Rabbit Polyclonal Antibody
Overview
| Product name: | KIF2C (phospho Ser95) rabbit pAb |
| Alternative Names: | KIF2C; KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK |
| Applications: | WB;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human KIF2C around the phosphorylation site of Ser95. AA range:61-110 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 80kD |
| GeneID(Human): | 11004 |
| Human Swiss-Prot No: | Q99661 |
| Cellular localization: | Cytoplasm, cytoskeleton . Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. . |
| Background: | kinesin family member 2C(KIF2C) Homo sapiens This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Species Reactivity: | Human;Mouse |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using KIF2C (Phospho-Ser95) Antibody -
Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from HeLa cells treated with TNF 10ng/ml 30', using KIF2C (Phospho-Ser95) Antibody. The lane on the right is blocked with the phospho peptide. -
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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