STAG3 Rabbit Polyclonal Antibody

Name: STAG3 Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES4546
Price: 248.0 USD
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$248.00 USD

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STAG3 Rabbit Polyclonal Antibody

Overview

Product name:	STAG3 rabbit pAb
Alternative Names:	STAG3; Cohesin subunit SA-3; SCC3 homolog 3; Stromal antigen 3; Stromalin-3
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human STAG3. AA range:1161-1210
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	139kD
GeneID（Human）:	10734
Human Swiss-Prot No:	Q9UJ98
Cellular localization:	Nucleus . Chromosome . Chromosome, centromere . Associates with chromatin. In prophase I stage of meiosis, it is found along the axial elements of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. It however remains chromatin associated at the centromeres up to metaphase I. During anaphase I, it probably dissociates from centromeres, allowing chromosomes segregation. .
Background:	The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014],
Species Reactivity:	Human;Rat;Mouse;

Western Blot analysis of Jurkat cells using STAG3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from Jurkat cells, using STAG3 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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STAG3 Rabbit Polyclonal Antibody

STAG3 Rabbit Polyclonal Antibody

Overview