CD133 Rabbit Polyclonal Antibody

Name: CD133 Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES3922
Price: 248.0 USD
Availability: InStock

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CD133 Rabbit Polyclonal Antibody

Overview

Product name:	CD133 rabbit pAb
Alternative Names:	PROM1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from the N-terminal region of human PROM1. AA range:41-90
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	100kD
GeneID（Human）:	8842
Human Swiss-Prot No:	O43490
Cellular localization:	Apical cell membrane ; Multi-pass membrane protein . Cell projection, microvillus membrane ; Multi-pass membrane protein . Cell projection, cilium, photoreceptor outer segment . Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
Background:	This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],
Species Reactivity:	Human;Mouse

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western Blot analysis of A549, SW620, HT29, Jurkat, mouse kidney, mouse lung cells using CD133 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30m
Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30m

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CD133 Rabbit Polyclonal Antibody

CD133 Rabbit Polyclonal Antibody

Overview