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ELK Biotechnology
SKU:ES3885
Catalase Rabbit Polyclonal Antibody
Catalase Rabbit Polyclonal Antibody
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$248.00 USD
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Catalase Rabbit Polyclonal Antibody
Overview
| Product name: | Catalase rabbit pAb |
| Alternative Names: | CAT; Catalase |
| Applications: | IF;WB;IHC;ELISA |
| Recommended Dilutions: | IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the C-terminal region of human CAT. AA range:478-527 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 60kD |
| GeneID(Human): | 847 |
| Human Swiss-Prot No: | P04040 |
| Cellular localization: | Peroxisome. |
| Background: | This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009], |
| Species Reactivity: | Human;Rat;Mouse;;Fish |
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Western blot analysis of lysates from 1) HT29, 2) A549, 3) HELA cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody(2B8) (cat:YM3029) antib -
Immunofluorescence analysis of human-liver tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Pi -
Immunofluorescence analysis of human-liver tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Pi -
Immunofluorescence analysis of human-kidney-cancer tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Ta
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