Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
Synthesized peptide derived from Myosin VI . at AA range: 40-120
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
149kD
GeneID（Human）:
4646
Human Swiss-Prot No:
Q9UM54
Cellular localization:
Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Golgi apparatus . Nucleus . Cytoplasm, perinuclear region . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Cell projection, filopodium . Cell projection, ruffle membrane . Cell projection, microvillus . Cytoplasm, cytosol . Also present in endocyctic vesicles (PubMed:16507995). Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage (PubMed:16507995). Recruited into membrane ruffles from cell surface by EGF-stimulation (PubMed:9852149). Colocalizes with DAB2 in clathrin-coated pits/vesicles (PubMed:11967127). Colocalizes with OPTN at the Golgi complex and in vesicul
Background:
myosin VI(MYO6) Homo sapiens This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014],
Western Blot analysis of extracts from Jurkat cells, using Myosin VI Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100