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ELK Biotechnology
SKU:ES3732
ZIS Rabbit Polyclonal Antibody
ZIS Rabbit Polyclonal Antibody
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$248.00 USD
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ZIS Rabbit Polyclonal Antibody
Overview
| Product name: | ZIS rabbit pAb |
| Alternative Names: | ZRANB2; ZIS; ZNF265; Zinc finger Ran-binding domain-containing protein 2; Zinc finger protein 265; Zinc finger; splicing |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ZNF265. AA range:106-155 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 34kD |
| GeneID(Human): | 9406 |
| Human Swiss-Prot No: | O95218 |
| Cellular localization: | Nucleus . |
| Background: | domain:The RanBP2-type zinc fingers mediate binding to RNA.,function:Splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. May interfere with constitutive 5'-splice site selection.,PTM:Isoform 2 is phosphorylated on Ser-310 upon DNA damage, probably by ATM or ATR.,sequence caution:Intron retention.,similarity:Belongs to the ZRANB2 family.,similarity:Contains 2 RanBP2-type zinc fingers.,subunit:Interacts with the C-terminal half of SNRNP70, the Arg/Ser-rich domain of XE7 as well as with U2AF1 and CLK1., |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using ZIS Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunofluorescence analysis of HUVEC cells, using ZNF265 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HeLa cells, using ZNF265 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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